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asci cleaved pctv vector  (Addgene inc)


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    Structured Review

    Addgene inc asci cleaved pctv vector
    Asci Cleaved Pctv Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/asci+cleaved+pctv+vector/CTV+(Plasmid+%2315912)/pmc13043292-226-52-56
    Average 93 stars, based on 41 article reviews
    asci cleaved pctv vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Cbfβ2 deficiency preserves Langerhans cell precursors by lack of selective TGFβ receptor signaling
    Article Snippet: .. To generate the Rosa26 Cbfb2 allele, cDNA encoding Cbfβ2 was amplified by PCR to add AscI sites at both ends, and this was ligated into an AscI-cleaved pCTV vector (15912l Addgene). .. C57/BL6 mice congenic for the CD45 locus were purchased from Sankyo Labo Service Coro.

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice
    Article Snippet: .. In order to construct the target vector for R26 lsl-R1 , R26 lsl-R1-WRPW , R26 lsl-R1-WRPF , R26 lsl-R3 and R26 lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice.
    Article Snippet: .. In order to construct the target vector for R26lsl-R1, R26lsl-R1-WRPW, R26lsl-R1-WRPF, R26lsl-R3 and R26lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems
    Article Snippet: .. To generate a Rosa26 LSL-OsTIR1 allele, cDNA encoding OsTIR1 F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). .. Subsequently, 30 μg of the target vector was linearized by AsiSI enzyme and was transfected into the M1 embryonic stem (ES) cell line by electroporation as previously described .

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems.
    Article Snippet: .. Generation of Rosa26LSL-OsTIR1 mouse strain To generate a Rosa26LSL-OsTIR1 allele, cDNA encoding OsTIR1F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). ..

    Polymerase Chain Reaction:

    Article Title: Cbfβ2 deficiency preserves Langerhans cell precursors by lack of selective TGFβ receptor signaling
    Article Snippet: .. To generate the Rosa26 Cbfb2 allele, cDNA encoding Cbfβ2 was amplified by PCR to add AscI sites at both ends, and this was ligated into an AscI-cleaved pCTV vector (15912l Addgene). .. C57/BL6 mice congenic for the CD45 locus were purchased from Sankyo Labo Service Coro.

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice
    Article Snippet: .. In order to construct the target vector for R26 lsl-R1 , R26 lsl-R1-WRPW , R26 lsl-R1-WRPF , R26 lsl-R3 and R26 lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice.
    Article Snippet: .. In order to construct the target vector for R26lsl-R1, R26lsl-R1-WRPW, R26lsl-R1-WRPF, R26lsl-R3 and R26lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems
    Article Snippet: .. To generate a Rosa26 LSL-OsTIR1 allele, cDNA encoding OsTIR1 F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). .. Subsequently, 30 μg of the target vector was linearized by AsiSI enzyme and was transfected into the M1 embryonic stem (ES) cell line by electroporation as previously described .

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems.
    Article Snippet: .. Generation of Rosa26LSL-OsTIR1 mouse strain To generate a Rosa26LSL-OsTIR1 allele, cDNA encoding OsTIR1F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). ..

    Plasmid Preparation:

    Article Title: Cbfβ2 deficiency preserves Langerhans cell precursors by lack of selective TGFβ receptor signaling
    Article Snippet: .. To generate the Rosa26 Cbfb2 allele, cDNA encoding Cbfβ2 was amplified by PCR to add AscI sites at both ends, and this was ligated into an AscI-cleaved pCTV vector (15912l Addgene). .. C57/BL6 mice congenic for the CD45 locus were purchased from Sankyo Labo Service Coro.

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice
    Article Snippet: .. In order to construct the target vector for R26 lsl-R1 , R26 lsl-R1-WRPW , R26 lsl-R1-WRPF , R26 lsl-R3 and R26 lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Runx/Cbfβ regulates the development of tolerogenic Thetis cells
    Article Snippet: .. This cDNA fragment was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice.
    Article Snippet: .. In order to construct the target vector for R26lsl-R1, R26lsl-R1-WRPW, R26lsl-R1-WRPF, R26lsl-R3 and R26lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems
    Article Snippet: .. To generate a Rosa26 LSL-OsTIR1 allele, cDNA encoding OsTIR1 F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). .. Subsequently, 30 μg of the target vector was linearized by AsiSI enzyme and was transfected into the M1 embryonic stem (ES) cell line by electroporation as previously described .

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems.
    Article Snippet: .. Generation of Rosa26LSL-OsTIR1 mouse strain To generate a Rosa26LSL-OsTIR1 allele, cDNA encoding OsTIR1F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). ..

    Construct:

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice
    Article Snippet: .. In order to construct the target vector for R26 lsl-R1 , R26 lsl-R1-WRPW , R26 lsl-R1-WRPF , R26 lsl-R3 and R26 lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice.
    Article Snippet: .. In order to construct the target vector for R26lsl-R1, R26lsl-R1-WRPW, R26lsl-R1-WRPF, R26lsl-R3 and R26lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Mutagenesis:

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice
    Article Snippet: .. In order to construct the target vector for R26 lsl-R1 , R26 lsl-R1-WRPW , R26 lsl-R1-WRPF , R26 lsl-R3 and R26 lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Article Title: Phosphorylation of Runx protein controls helper CD4 + T cell versus cytotoxic CD8 + T cell lineage choice.
    Article Snippet: .. In order to construct the target vector for R26lsl-R1, R26lsl-R1-WRPW, R26lsl-R1-WRPF, R26lsl-R3 and R26lsl-R3-WRPW mice, cDNA encoding WT and mutant Runx1 and Runx3 proteins were amplified by PCR to add AscI sites at the both ends, and these DNA fragments was ligated into an AscI-cleaved pCTV vector (#15912, Addgene). ..

    Introduce:

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems
    Article Snippet: .. To generate a Rosa26 LSL-OsTIR1 allele, cDNA encoding OsTIR1 F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). .. Subsequently, 30 μg of the target vector was linearized by AsiSI enzyme and was transfected into the M1 embryonic stem (ES) cell line by electroporation as previously described .

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems.
    Article Snippet: .. Generation of Rosa26LSL-OsTIR1 mouse strain To generate a Rosa26LSL-OsTIR1 allele, cDNA encoding OsTIR1F74G was amplified by PCR to introduce AscI sites at the both ends, and inserted into an AscI-cleaved pCTV vector (Addgene, 15912). ..



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    93
    Addgene inc asci cleaved pctv vector
    Asci Cleaved Pctv Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/asci+cleaved+pctv+vector/CTV+(Plasmid+%2315912)/pmc13043292-226-52-56
    Average 93 stars, based on 1 article reviews
    asci cleaved pctv vector - by Bioz Stars, 2026-10
    93/100 stars
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